연구
Research Outcome
미래를 창조하는 포스텍 화학공학과
Pathway optimization by re-design of untranslated regions for L-tyrosine production in Escherichia coli
- Title of paper
- Pathway optimization by re-design of untranslated regions for L-tyrosine production in Escherichia coli
- Author
- [정규열교수 연구실]
- Publication in journal
- Scientific Reports 5, pp13853 (September 2015)
- Publication date
- 20150908
[Abstract]
L-tyrosine is a commercially important compound in the food, pharmaceutical, chemical, and cosmetic industries. Although several attempts have been made to improve L-tyrosine production, translation-level expression control and carbon flux rebalancing around phosphoenolpyruvate (PEP) node still remain to be achieved for optimizing the pathway. Here, we demonstrate pathway optimization by altering gene expression levels for L-tyrosine production in Escherichia coli. To optimize the L-tyrosine biosynthetic pathway, a synthetic constitutive promoter and a synthetic 5′-untranslated region (5′-UTR) were introduced for each gene of interest to allow for control at both transcription and translation levels. Carbon flux rebalancing was achieved by controlling the expression level of PEP synthetase using UTR Designer. The L-tyrosine productivity of the engineered E. coli strain was increased through pathway optimization resulting in 3.0 g/L of L-tyrosine titer, 0.0354 g L-tyrosine/h/g DCW of productivity, and 0.102 g L-tyrosine/g glucose yield. Thus, this work demonstrates that pathway optimization by 5′-UTR redesign is an effective strategy for the development of efficient L-tyrosine-producing bacteria.
doi: 10.1038/srep13853
37673 경북 포항시 남구 청암로 77 포항공과대학교 화학공학과 (환경공학동) ㅣ전화 : 054-279-2720 ㅣ팩스 : 054-279-5528
Copyright © chemical engineering. All rights reserve
본 사이트는 IE 상위버전에 최적화 되어있습니다.